TissueFAXS Plus

TissueQuest is the first software solution worldwide to identify and analyse individual cells in their tissue environment

Cell identification is not confined to isolated cells. The TissueQuest software also identifies cells that are agglomerated in dense clusters or have been reduced to ring structures (as it is typical of cancer cells).

Regardless of the type and density of cells involved, TissueQuest recognizes their nuclei due to patented image processing algorithms. By combining specific stainings and algorithms, one can measure staining intensities that are specific for various cell types.

Cells are clearly distinguished even:

-in solid tissue structures
-if embedded in clusters
-if nuclei are uniformly stained
-if staining involves ring structures

Analysis of staining intensities can be restricted to the area of the nucleus or can be extended into the cytoplasm. In case of cytoplasmic antigens TissueQuest provides an image processing algorithm that is growing in stained areas around the nucleus.

Backward Gating enables the user to connect backwards from a dot in the dotplot to the specific corresponding cell in the image. By Forward Gating the user is able to locate the dot in the plot corresponding to a specific cell in the image. The function “View event data” reads out all measurement data for an individual cell.

-TissueQuest can load complete TissueFAXS projects or import any series of .tif or .jpg images for analysis
-Powerful image processing algorithms for the identification of single cells in tissue based on nuclear staining
-Automatic generation of ”cell body” (cytoplasmatic) masks for intensity measurements
-Complete set of manual modification and correction tools (splitting, merging, deleting and creating nuclei)
-Powerful DotPlot operations -Forward Gating of cells to the DotPlots and Backward Gating of dots, gates or cutoff quadrants to the image
-Unlimited custom DotPlot and histogram generation, also based on gates in existing DotPlots, allow for near-unlimited new analyses and refinement of existing ones
-Extensive printed report and data export options